The free-living flatworm, Macrostomum lignano has an impressive regenerative capacity. Following injury, it can regenerate almost an entirely new organism because of the presence of an abundant somatic stem cell population, the neoblasts. This set of unique properties makes many flatworms attractive organisms for study-ing the evolution of pathways involved in tissue self-renewal, cell-fate specification, and regeneration. The use of these organisms as models, however, is hampered by the lack of a well-assembled and annotated genome sequences, fundamental to modern genetic and molecular studies. Here we report the genomic sequence of
2016
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10
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24
Whole genome protein families were classified by InterProScan analysis (Jones et al., 2014). The peptidase families were identified by BLASTP searching against MEROPS peptidase database release 9.12 with a cutoff e-value of 1e-20 (Rawlings et al., 2014). The transporters were classified on the basis of Transport Classification Database (Saier et al., 2014). Carbohydrate-active enzymes were classified by using hmmer 3.0 (Mistry et al., 2013) to search against a library of catalytic and carbohydrate-binding module enzymes acquired from dbCAN (Yin et al., 2012). G-protein-coupled receptors were selected from the best hits with GPCRDB sequences (Horn et al., 2003) and by confirming that they contained seven transmembrane helices with the amino terminus outside and the carboxyl terminus inside the plasma membrane. Homologs of the Pth11-like GPCRs in Magnaporthales (Kulkarni et al., 2005) were identified by local BLASTP analysis with a cutoff e-value of 1e-10. The divergence tim...
2013
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11
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